Mass Cytometry
About Mass Cytometry
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Cytometry by time-of-flight (CyTOF®), or generically, Mass Cytometry(MC) is a hybrid technology of flow cytometry and mass spectrometry to detect metal-conjugated antibodies that are bound intracellularly or extracellularly to antigens of interest on single cells.
Advantages of core technology over existing technology problems
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Since 2018, the application of immunophenotyping based on mass cytometry to immune cells has increased rapidly in the analysis of immune responses related to autoimmune diseases, viral infectious diseases, cancer, and personalized medicine. (Biology 2021, 10(2), 104)
Fluorescence labeling: Proteome analysis restricted due to signal overlap.
Mass labeling: High resolution of analysis makes it suitable for multiplex proteome analysis.
| 기존 기술 (유세포 분석법) |
구분 | 핵심 기술 (질량 세포 분석법, CyTOF) |
|---|---|---|
| ~20 |
|
40개 이상 |
| 많이 필요 |
혈액 샘플
|
적은 양으로 측정 가능 |
| 보정 필요 |
신호간섭
|
보정 불필요 |
| 상대값 적용 측정시마다 오차 발생 큼 |
측정결과
|
절대값 적용 오차가 적음 |
| 낮음 (특히 rare population) |
민감도
|
높음 (특히 rare population) |
| 2차원 |
Data presentation
|
다차원 |
| 높음 |
시험간 variation
|
낮음 |
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Mass cytometry (CyTOF) allows to assess virus specific T cells
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(Examples of R&D results) SARS-CoV2 specific T cells can be detected by mass cytometry technology
